Coding

Part:BBa_K1975005

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-10-05)


Cyan Fluorescent Protein

This is Cyan Fluorescent Protein that can be used to tag proteins of interest. It is a fluorescent protein that will show a blue color when it is excited at 405nm and it has the highest absorbance at 485nm. This sequence has been used to tag PhaA to visualize the expression of the protein. If the protein was expressed correctly, the cells would show a blue color when excited. The sequence is optimized for use in Bacillus subtilis.

  • Cfp is a powerful tool for detecting nanoscale and nanoscale distance changes in biological macromolecules in vivo. It can be used to detect the direct interaction of two protein molecules in a cell. When FRET occurs in CFP/YFP, the energy of CFP is transferred to YFP when YFP is excited by 440nm light. When the YFP is bleached, the energy transfer process of CFP to YFP is interrupted. The weaker CFP became brighter, confirming FRET.

Experimental Results

Improved Results

  • Group: LZU-CHINA 2019
  • Author: Jian Qi; Haodong Yan
  • Summary: We linked cyan fluorescent protein to the Ras-Raf system to detect the interaction between the two proteins. We use this part to do the experiment.
T--LZU-CHINA--cfp.png
T--LZU-CHINA--Flu.png
  • Imaging begins approximately 2 hours after the media is replaced with a fluorescent imaging medium. Get images every 1 hour. SW1990 cells were observed using 440 nm excitation light, and the cells turned from cyan to yellow.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None